Plasmid

Part:BBa_K1401606:Design

Designed by: Yash Agarwal   Group: iGEM14_BostonU   (2014-10-11)


pBad- BCD2 - RFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 129
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 69
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 805
    Illegal AgeI site found at 917
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

All basic BioBricks used were transformed into MoClo variants by adding the following fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. These were then put together using the digestion-ligation MoClo reaction.


Source

E. Coli

References